PCR cloning requires an existing template, such as a cDNA library or genomic DNA. Gene synthesis chemically assembles a sequence directly from nucleotide monomers and does not require a template. It is suitable for rare genes or genes without an available template, sequences requiring extensive codon optimization, artificially designed synthetic-biology elements, and sequences containing toxic or difficult-to-amplify structures.

Yes. Based on the protein amino acid sequence, we can perform reverse translation and sequence optimization by considering the codon preferences of the target host, GC content, repetitive sequences, secondary structure, and other factors, then design and synthesize a DNA sequence suitable for expression.

For standard sequences, turnaround can be as fast as 1–7 calendar days for fragments under 400 bp, about 7–10 days for fragments under 1.2 kb, about 10–15 days for 1.2–2 kb, about 15–20 days for 2–3 kb, and about 25–30 days for 4.5–5 kb. Sequences longer than 5 kb and difficult sequences with high GC content or repeats require evaluation before the turnaround time and quotation can be confirmed.

Yes. Before synthesis, we provide a complimentary sequence assessment covering GC content, repetitive sequences, secondary structure, and toxicity prediction. Strategies may include codon optimization, segmented synthesis, switching to a low-copy vector, and using specialized host strains. The assessment report is provided within one business day.

Before delivery, each synthesized gene is verified by Sanger sequencing and aligned against the confirmed reference sequence. The original .ab1 chromatogram files and a sequence alignment report are included. If a quality issue is confirmed to have been caused by us, the gene will be resynthesized at no additional charge in accordance with the agreed terms.

More than 200 vectors are available at no additional cost, including prokaryotic expression vectors (pET, pGEX, pMAL, pCold, and pBAD), mammalian expression vectors (pcDNA3.1, pCMV, pEF, pIRES, and lentiviral systems), cloning vectors (pUC57/19/18, pBluescript, and T vectors), as well as yeast and insect vectors. We can also use a vector supplied by you.

Yes. Customer-provided sequences and project information are kept strictly confidential and will not be disclosed to any third party or used for any other purpose without written authorization. A non-disclosure agreement (NDA) can be signed upon request.