Language
中文 English
DNA Transfection Reagent
ZY FectEasy DNA Transfection Reagent uses cationic liposome technology and is available in standard and high-performance versions. It combines high efficiency, low cytotoxicity, and serum compatibility for plasmid DNA delivery into both routine and difficult-to-transfect cells.
Consult Now

  • 2versions

    Standard and high-performance options

  • 72h

    No obvious cytotoxicity in most cells

  • 1:1-1:2.5

    Recommended DNA-to-reagent ratio

  • 1year

    Shelf life at 4°C

首页 > Products > Molecular Biology Reagents > DNA Transfection Reagent >
Product Overview
Ordering Information
Product Advantages
Product Specifications and Applications
Procedure
Recommended Dosage and Setup
Applications
Frequently Asked Questions
Related Products and Services
Series Overview
Standard Version
Enhanced Version

ZY FectEasy DNA Transfection Reagent Series

Dual selection of standard/enhanced, high efficiency and low toxicity, serum compatible

The ZY FectEasy DNA Transfection Reagent series uses advanced cationic liposome technology and is specifically designed for efficient delivery of plasmid DNA and nucleic acid-protein complexes into eukaryotic cells.


The standard version meets the needs of common adherent and suspension cells; the enhanced version optimizes the lipid formulation for higher efficiency in hard-to-transfect cell lines.


Both products are easy to use, serum-compatible, and low in cytotoxicity, suitable for gene overexpression, gene function verification, and cell model construction.

ZY FectEasy DNA Transfection Reagent Series

ZY FectEasy DNA Transfection Reagent (Standard Version)

Efficient plasmid DNA delivery for common adherent and suspension cells

Based on cationic liposome technology, plasmid DNA and nucleic acid-protein complexes can be transfected into cultured eukaryotic cells. It has high transfection efficiency, good reproducibility, and easy operation; most cells show no significant toxicity within 72 hours without changing the culture medium, and the presence of serum does not affect efficiency.

Suitable for adherent and suspension cells, compatible with serum-containing culture medium.

  • High transfection efficiency, good reproducibility, simple operation steps.
  • Low cytotoxicity, most cells show no obvious cytotoxicity within 72 hours after transfection without changing the culture medium.
  • Wide applicability, can be used for both adherent and suspension cells, compatible with serum-containing culture medium.
ZY FectEasy DNA Transfection Reagent Standard Version

ZY FectEasy Plus DNA High-Efficiency Transfection Reagent

Upgraded liposome formula for hard-to-transfect cells

ZY FectEasy DNA High-Efficiency Transfection Reagent is an enhanced version of the ZY FectEasy series, using an optimized cationic liposome formula that enables higher levels of plasmid DNA delivery in both common and hard-to-transfect cell lines. Compared with conventional DNA transfection reagents, it can further reduce reagent usage while maintaining low cytotoxicity, improving transfection efficiency and experimental economy.

  • High-Efficiency Delivery: Optimized for hard-to-transfect cell lines, with transfection efficiency further improved compared to the conventional version
  • Low Toxicity and Gentle: Cells remain in good condition after transfection, with most cells showing no significant toxicity within 72 hours
  • Broad applicability: suitable for adherent cells and suspension cells
  • Flexible experiment: can be operated in serum-containing medium, simplifying the process
ZY FectEasy Plus DNA High-Efficiency Transfection Reagent

Ordering Information

Flexible choice between standard version and high-efficiency version
Product
Name		 Catalog No. Size Applicable
Scene		 Typical
Cells		 Price
ZY FectEasy
DNA Transfection Reagent		 ZY-FE-D1000 1mL Common adherent/
Suspension cell plasmid transfection,
Pursuit of cost-effectiveness		 HEK293/293T、H
eLa、HCT116、A549
Contact Us
ZY FectEasy Plus
DNA high-efficiency transfection reagent		 ZY-FEP-D1000 1mL Hard-to-transfect cell lines or
Higher transfection efficiency required		 Primary cells, stem cells,
Some tumor cells
Contact Us

Product Advantages

High efficiency, low toxicity, broad spectrum, flexible
  • Efficient delivery
    The standard version has high transfection efficiency and good reproducibility; the enhanced version is optimized for hard-to-transfect cells, further improving efficiency.
  • Low toxicity and gentle
    Cells remain in good condition after transfection, and most cells show no obvious cytotoxicity even if the culture medium is not changed within 72 hours.
  • Broad Spectrum Application
    Can be used for common and hard-to-transfect cells, as well as adherent and suspension cells.
  • Serum Compatible
    Can be operated in serum-containing media to simplify experimental procedures; the enhanced version can also reduce reagent usage.

Product Specifications and Applications

Suitable for a variety of DNA delivery experiments
Project Parameters
Product Name ZY FectEasy DNA Transfection Reagent / ZY FectEasy Plus DNA High-Efficiency Transfection Reagent
Size 1 mL
Storage and Transportation Store at 4°C, transport with ice packs
Shelf Life 1 year
Applicable Nucleic Acids Plasmid DNA, nucleic acid-protein complexes, etc.
Scope of Application Specific scenarios
Common cell lines Plasmid DNA transfection in HEK293, HeLa, A549, HepG2, etc.
Hard-to-transfect cells Gene delivery in some primary cells, tumor cells, suspension cells
Reporter genes Transfection of expression vectors such as GFP, Luciferase
Gene editing Delivery of tools such as CRISPR/Cas9 vectors, TALENs
Functional studies Gene overexpression, co-transfection of multiple plasmids

Procedure

Using a 6-well plate as an example
  • Plating and Medium Change
    Seed the day before; at the time of transfection, confluency should be 50%–70%; about 0.4×10⁶ 293T cells per well. Replace with 2 mL of fresh complete medium before transfection.
  • Dilute separately
    Add 4 μg plasmid DNA to 250 μL DMEM; in another tube, add 10 μL reagent to 250 μL DMEM, mix gently, and let stand at room temperature for 5 min.
  • Prepare and add the complex
    Mix equal volumes, mix gently, and incubate at room temperature for 20 min; add 500 μL DNA-liposome complex evenly to the cells and gently shake to mix.
  • Culture and detection
    After 6–12 h, replace with fresh complete medium, continue culture for 24–40 h, and detect expression. The complex should not be vortexed or centrifuged; antibiotics are recommended to be removed for sensitive cells.

Recommended Dosage and Setup

The suggested ratio of DNA to reagent is 1:1–1:2.5
Culture vessels Surface area Culture medium volume Amount of DNA ZY FectEasy DNA ZY FectEasy DNA Potent
96-well 0.3 cm² 100 μL 0.2 μg 0.2–0.5 μL 0.2–0.5 μL
48-well 0.8 cm² 350 μL 0.5 μg 0.5–1.25 μL 0.5–1.25 μL
24-well 2 cm² 500 μL 0.8 μg 0.8–2 μL 0.8–2 μL
12-well 4 cm² 1 mL 1.6 μg 1.6–4 μL 1.6–4 μL
6-well 10 cm² 2 mL 4.0 μg 4–10 μL 4–8 μL
60 mm 20 cm² 4 mL 8.0 μg 8–20 μL 8–20 μL
100 mm 60 cm² 12 mL 24 μg 24–60 μL 24–60 μL

Note:The recommended ratio of DNA (μg) to reagent (μL) is 1:1–1:2.5; the specific amount needs to be optimized according to the cell type, plasmid size, and culture conditions.

Applications

From conventional cells to hard-to-transfect cells
Transfection of common cell lines

Transfection of common cell lines

Common plasmid DNA delivery for HEK293/293T, HeLa, A549, HepG2, and other conventional cell lines

Delivery to Hard-to-Transfect Cells

Delivery to Hard-to-Transfect Cells

Primary cells, stem cells, certain tumor cells, and suspension cells

Reporter Gene Expression

Reporter Gene Expression

Transfection and detection of reporter vectors such as GFP, Luciferase

Delivery of Gene Editing Tools

Delivery of Gene Editing Tools

CRISPR/Cas9 vectors, TALEN, and other genome editing tools

Co-transfection of Multiple Plasmids

Co-transfection of Multiple Plasmids

Gene overexpression, functional validation, and cell model construction

Frequently Asked Questions FAQ

Common Issues in DNA Transfection Experiments
What is the appropriate cell density before transfection?
For adherent cells, 50%–70% confluence is recommended; for suspension cells, a density of 2×10⁶–4×10⁶ cells/mL. Both overly high and overly low densities will reduce efficiency.
Is serum-free medium required during transfection?
It is recommended to use serum-free medium when diluting DNA and transfection reagents; adding serum-containing medium after the complex is formed usually does not affect efficiency.
Can antibiotics be added to the medium?
Antibiotics may reduce activity by increasing cell permeability, so it is recommended to avoid using them in the transfection system.
What should I do if transfection efficiency is low?
Check cell condition, plasmid purity (OD₂₆₀/OD₂₈₀ ≥ 1.8), and endotoxin levels, and optimize the DNA/reagent ratio and cell density.
What causes cell death after transfection?
Common reasons include poor cell condition, impure plasmid, high endotoxin levels, excessive reagent, or untimely medium change. It is recommended to adjust the amounts and replace with fresh medium promptly.

Related Products and Services

From vector construction to high-quality plasmid preparation

Copyright © Shanghai ZhiYin Biotechnology Co., Ltd. All Rights Reserved.