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DNA Marker

The ZY RulerStar Prestained DNA Marker covers 100 bp to 12 kb and is premixed with 1× Loading Buffer and GelRed for direct loading at 3–5 μL.
It is suitable for PCR, restriction digestion, cloning screening, and vector construction verification.

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4^formats
Coverage for diverse applications
100bp-12kb
Fragment range
3-5^μL
Direct loading
2^years
Long-term storage at –20°C

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Product Overview

Ordering Information

Product Highlights

Product Specifications

Components and Storage

Procedure

Applications

Frequently Asked Questions

ZY RulerStar Pre-stained DNA Marker

Covers the full range from 100 bp to 12 kb

ZY RulerStar Pre-stained DNA Marker is a pre-stained, ready-to-use DNA ladder launched by Zhi Yin Biologicals for molecular biology research, specifically designed for agarose gel electrophoresis. The product is composed of a mixture of independent linear double-stranded DNA fragments derived from digested plasmids, pre-mixed with 1× Loading Buffer, and embedded with GelRed nucleic acid dye.

Directly load 3–5 μL without additional staining; bands are clearly visible under a UV lamp, eliminating the need for post-staining with EB/SYBR and the issues of exposure shielding. The series includes four specifications, covering mainstream applications from 100 bp to 12 kb.

ZY RulerStar Pre-stained DNA Marker Gel Electrophoresis

Ordering Information

4 specifications covering 100 bp–12 kb

Product Name	Size	Catalog No.	Product Information	Price
ZY RulerStar 1 kb-IV DNA Marker	250 μL （50 lanes）	ZY-RS1000IV-250	13 bands · 250 bp–12 kb	Contact Us
ZY RulerStar 100 bp-II plus DNA Marker	500 μL （100 lanes）	ZY-RS100II-500	11 bands · 100 bp–1500 bp	Contact Us
ZY RulerStar 250 bp-I DNA Marker	500 μL （100 lanes）	ZY-RS250I-500	6 bands · 100 bp–2000 bp	Contact Us
ZY RulerStar 250 bp-II DNA Marker	500 μL （100 lanes）	ZY-RS50II-500	9 bands · 100 bp–5000 bp	Contact Us

Product Highlights

Clear, stable, ready-to-use

Ready to run, stain-free imaging

Pre-mixed 1× Loading Buffer + GelRed, load 3–5 μL directly for electrophoresis, no need to mix gel with dye, and photograph under UV immediately after electrophoresis.
Clear bands, accurate determination

Enzyme-digested purified fragments, clean background; 1–2 enhanced reference bands (2.5× concentration) for visual positioning, also suitable for semi-quantitative analysis.
Complete specifications, full coverage of scenarios

4 types of markers covering 100 bp–12 kb, meeting daily experimental needs such as PCR, restriction digestion, clone screening, and reporter gene detection.
Stable storage, resistant to handling

Store at 4°C for 6 months, –20°C for 2 years; performance remains unchanged after ≤5 freeze-thaw cycles, accelerated validation at 37°C passed.
Strong dye compatibility

Compatible with mainstream nucleic acid dyes such as GelRed, GelGreen, SYBR Safe, SYBR Green I; suitable for pre-staining or post-staining.

Product Specifications

Select the corresponding specification according to the experimental fragment range.

Product Name	Electrophoresis Reference Image	Number of Bands	Fragment Range	Highlight Reference ladder
1 kb-IV DNA Marker		13	250 bp–12 kb	1500 / 4000 bp（2.5×）
100 bp-II plus DNA Marker		11	100 bp–1500 bp	500 bp（2.5×）
250 bp-I DNA Marker		6	100 bp–2000 bp	750 bp（2.5×）
250 bp-II DNA Marker		9	100 bp–5000 bp	500 / 1500 bp（2.5×）

Product Name	Recommended Gel concentration	Product Characteristics	Applicable to Scenario
1 kb-IV DNA Marker	1.0% Agarose / 1×TAE	Wide spacing and large-range design, Covers PCR small products to 12 kb large fragments	cDNA / ORF clone insert fragment screening, Long fragment CRISPR vector construction verification
100 bp-II plus DNA Marker	1.5–2.0% Agarose or 5% PAGE / 0.5×TBE	High-density band design, Suitable for precise confirmation of DNA fragment size	qPCR, miRNA precursor cloning, saRNA/siRNA double-strand annealing, Indel detection
250 bp-I DNA Marker	1.0–2.0% Agarose / 1×TAE preferred or 0.5×TBE	Few bands, large spacing, Suitable for rapid analysis of PCR and restriction enzyme products	Conventional PCR, shRNA stem-loop PCR, Validation of siRNA template oligo annealing products
250 bp-II DNA Marker	1.0–2.0% Agarose / 1×TAE preferred or 0.5×TBE	Wide spacing, large range specifications, Coverage up to 5 kb	Reporter gene vectors, shRNA expression clones, gRNA expression vector digestion analysis

Components and Storage

Ready-to-use upon opening, stably preserved

Product Composition	Detailed Content
Premixed System	1×Loading Buffer：10 mM Tris-HCl pH 7.6、5 mM EDTA、 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, GelRed nucleic acid dye, ready-to-use upon opening.
Included Components	5×Loading Buffer (with dye) for sample preparation.
Usage Recommendations	Aliquot according to the number of lanes (e.g., 50 μL/tube), Avoid repeated freeze-thaw cycles; mix well after thawing before use.

Storage Conditions	Temperature	Shelf Life
Short-term / daily use	4°C	≥6 months
Long-term storage	–20°C (avoid repeated freezing and thawing)	2 years
Transportation conditions	4°C ice pack / low-temperature transport in foam box	—

Procedure

Complete pre-stained DNA marker electrophoresis in four steps

Gel Preparation

Select the corresponding gel concentration according to the target fragment; no nucleic acid dyes need to be added.
Loading

Take the marker out from 4°C, let it equilibrate to room temperature, mix gently, and load 3–5 μL per lane directly.
Electrophoresis

Use 1×TAE or 0.5×TBE, 4–10 V/cm (7 V/cm recommended), until the front dye migrates to 2/3 of the gel.
Imaging

Directly place the gel under UV light to observe; the bands appear orange-red (GelRed channel), no post-staining required.

Applications

Covers common molecular biology experiments

PCR product verification

Determination of the size of conventional PCR products and small fragments.

Enzyme digestion and clone screening

Plasmid enzyme digestion, cDNA / ORF clone insertion fragment screening.

Vector Construction Validation

CRISPR, shRNA, and gRNA expression vector construction and restriction enzyme analysis.

Accurate Reading of Small Fragments

qPCR, miRNA precursors, saRNA/siRNA duplex annealing, and Indel detection.

Frequently Asked Questions

DNA Marker usage and storage Q&A

Is ZY RulerStar DNA Marker compatible with GelRed / GelGreen / SYBR dyes?

Yes. The product is pre-stained with GelRed and is also compatible with GelGreen, SYBR Safe, and SYBR Green I. For post-staining, please follow the dye manufacturer's procedures; during pre-staining, it is recommended to avoid adding extra nucleic acid dye to the gel, as it may affect the migration of small fragments.

What is the recommended loading amount for each lane? What happens if too much is loaded?

Recommended 5 μL per lane; for wide wells, it can be increased to 8–10 μL. Too much can cause band smearing and residual sample in the well; too little will result in weak signals for small bands.

How long can it be stored at 4°C? What is the effect of repeated freeze-thaw?

Stable for ≥6 months at 4°C; long-term storage at –20°C can last up to 2 years. It is recommended to aliquot; repeated freeze-thaw more than 5 times may cause small fragment degradation and reduced intensity of the bright bands.

Can the supplied 5×Loading Buffer be replaced with a regular product?

No. The marker itself contains GelRed; if the sample requires a dye, please use the 5×Loading Buffer provided with the product (contains dye).

What is the function of the bright reference band?

The bright band has a concentration 2.5 times that of the other bands, serving both as a visual positioning anchor and semi-quantitative reference; when the sample brightness is close to that of the bright band, the DNA concentration can be roughly estimated as 50–125 ng/5 μL.

Why is 100 bp-II plus recommended to be used with 0.5× TBE + 1.5–2.0% gel?

The 100 bp range bands are dense, requiring a high-concentration gel to ensure resolution; TBE provides better resolution than TAE for small fragment separation.

Is it normal for precipitate or turbidity to appear before use?

After long-term storage at 4°C, a small amount of EDTA salt precipitation or GelRed micro-aggregation is normal. Re-dissolve in a 37°C water bath for 5 min and gently mix.

Why does the marker float out of the loading well?

Common reasons include insufficient glycerol ratio, excessive use of buffer leading to decreased ionic strength, or excessive sample volume and insufficient density.

Can Marker be used on PAGE gels?

100 bp-II plus is recommended for 5% PAGE gels + 0.5×TBE; the other three types are mainly for agarose gels and are not recommended for PAGE.

4formats

100bp-12kb

3-5μL

2years

ZY RulerStar Pre-stained DNA Marker

Ordering Information

Product Highlights

Product Specifications

Components and Storage

Procedure

Applications

PCR product verification

Enzyme digestion and clone screening

Vector Construction Validation

Accurate Reading of Small Fragments

Frequently Asked Questions

Related Products and Services

4^formats

3-5^μL

2^years