ZY GelStar Gel Extraction Kit

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Gel Extraction Kit

The ZY GelStar Gel Extraction Kit combines a specialized silica membrane with an efficient gel-dissolving system to rapidly recover high-quality DNA from 100 bp to 9 kb from TAE/TBE agarose gels or PCR products.
Each column binds up to 10 μg DNA, with a maximum recovery rate of ≥80% and a minimum processing time of 13–18 minutes.

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13-18^min
Fastest processing time
100 bp-9 kb
Standard recovery range
≥ 80%
Maximum recovery rate
10 μg
Maximum binding capacity per column

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Product Overview

Ordering Information

Product Highlights

Product Specifications and Components

Procedure

Storage, Shipping, and Scope of Application

Frequently Asked Questions

Rapid recovery of high-quality DNA fragments from 100 bp–9 kb

The specially treated silica membrane and efficient sol-gel system allow for simple and rapid recovery of high-quality DNA from TAE or TBE agarose gels. Each adsorption column can specifically bind up to 10 μg of DNA, without adsorbing enzymes, mineral oil, or other impurities.

The recovery rate for 100 bp–9 kb fragments is about 50%–80%, with a maximum exceeding 80%; the recovered DNA can be directly used for restriction digestion, ligation, transformation, PCR templates, sequencing, and library construction.

The entire purification process takes as little as 13–18 minutes.
All steps are performed at room temperature, with no ice bath required.
Special silica membranes show minimal batch-to-batch variation and good reproducibility.

Direct purification of PCR products.

A dual-use box, purify PCR amplification products without cutting the gel

Can purify DNA fragments directly from the PCR amplification system. After adding 5 times the volume of binding buffer onto the column, high-purity products can be obtained through adsorption, washing, and elution.

Suitable for various DNA samples such as PCR products, plasmid restriction fragments, ligation products, and linearized plasmids. Purified products can be used immediately for downstream experiments or stored at -20°C.

Maximum adsorption capacity per column: 10 μg
Recommended elution: 30–50 μL
Compatible with routine enzymatic reactions and sequencing applications

Ordering Information

Available in 50-use and 200-use specifications, suitable for different experimental scales

Product Name	Catalog No.	Size	Applicable Scenarios	Price
ZY GelStar Gel Extraction Kit	ZY-GS-50	50 rxn	Small-scale experiments, method validation	Contact Us
ZY GelStar Gel Extraction Kit	ZY-GS-200	200 rxn	Routine molecular cloning, sample preparation before sequencing	Contact Us

Product Highlights

Fast, broad-spectrum, high-recovery, and highly compatible DNA purification solution

Quick and simple
The entire purification process takes as little as 13–18 minutes, operates entirely at room temperature, and requires no ice bath
Dual-purpose per box
Can be used both for agarose gel slice recovery and direct purification of PCR amplification products
Wide recovery range
Achieves ideal recovery efficiency for fragments ranging from 100 bp to 9 kb
High recovery rate
The maximum recovery rate can reach over 80%, and the recovered DNA can be directly used for downstream enzymatic reactions
Good stability
Specially treated silica membrane has minimal batch-to-batch differences, ensuring good experimental reproducibility
High compatibility
Suitable for PCR products, plasmid restriction fragments, ligation products, and linearized plasmids

Product Specifications and Components

Core performance parameters and components for 50- and 200-use packages

Item	Parameter
Recovery fragment range	100 bp–9 kb (recovery rate for 10–15 kb fragments approximately 20%–50%)
Maximum recovery rate	≥80%
Maximum adsorption per column	10 μg
Operation time	13–18 minutes
Storage Conditions	Stable at room temperature (15–25℃) for 1.5 years; long-term storage at 2–8℃
Transportation Conditions	Transport at room temperature
Purification Method	Silica gel membrane centrifuge column method

Components	ZY-GS-50	ZY-GS-200
Gel Recovery Column	50 pieces	200 pieces
2 mL Collection Tube	50 pieces	200 pieces
Binding Solution	25 mL	100 mL
Wash Solution (Concentrate)	12 mL	2×24 mL
Elution Buffer	10 mL	25 mL
Buffer CBS	15 mL	50 mL

Wash Solution: Before first use, add 4 volumes of absolute ethanol as indicated on the bottle and label it; binding solution should avoid direct strong light; elution buffer is recommended to be aliquoted to reduce contamination.

Procedure

Supports both agarose gel DNA recovery and direct purification of PCR products.

Part 1 Agarose Gel DNA Recovery

Gel cutting, gel dissolving, binding, washing, and elution

Gel Dissolving

Cut the target band and weigh it; add 150 μL of binding buffer per 100 mg of 1% gel, 55°C until completely melted
Load onto column for binding

Transfer to the gel recovery column, stand at room temperature for 2 minutes, centrifuge at 8000 rpm for 1 minute
Wash to remove alcohol

Wash twice with 500 μL of wash solution; centrifuge the empty column at 12000 rpm and open the lid to evaporate ethanol
Elution and recovery

Add 30–50 μL of preheated elution buffer to the center of the membrane, incubate at 37°C for 2 minutes, then centrifuge at 12000 rpm for 1 minute

Part 2 PCR product purification

Binding, adsorption, washing, elution, and detection

Add binding buffer

Add PCR reaction solution to 5 times the volume of binding buffer and mix thoroughly
Load onto column for binding

Transfer to the gel recovery column, stand at room temperature for 2 minutes, centrifuge at 8000 rpm for 1 minute
Wash to remove alcohol

Wash twice with 500 μL washing solution; centrifuge the empty column and leave the lid open for 10 minutes
Elution detection

Add 30–50 μL preheated elution buffer, centrifuge after 2 minutes at 37°C; take 2–5 μL for electrophoresis detection

Storage, Shipping, and Scope of Application

Stable storage and transport, compatible with common DNA purification scenarios

Room temperature storage
Dry storage at 15–25℃ is stable for 1.5 years.
Long-term storage
When the ambient temperature is too high or for long-term storage, keep at 2–8℃.
Transportation Conditions
Supports room temperature transport; if precipitation occurs at low temperature, return to room temperature, and if necessary, dissolve with a 37℃ water bath.
Conventional fragments
Recovery rate of 100 bp–9 kb fragments is about 50%–80%.
Long fragments
10–15 kb recovery rate is about 20%–50%, it is recommended to increase the sample amount, elute with 50 μL and heat elute at 65°C.
Scope of Use
For research use only, not for clinical diagnosis, treatment, food or drugs.

Frequently Asked Questions

Common questions about recovery rate, binding, elution, and downstream reactions

What should I do if the gel recovery rate is low?

Check whether ethanol has been added to the wash solution; if the sol liquid turns orange-red or pink (pH > 7.5), add 10 μL of 3 M NaAc (pH 5.0) to adjust it back to light orange-yellow; it is recommended to use freshly prepared TAE/TBE. For long fragments, you can increase the loading volume, increase the elution volume to 50 μL, and use 65°C heating for elution.

What should be done if PCR product purification efficiency is low?

Add a binding solution equivalent to 5 times the volume of the PCR system and mix thoroughly; confirm that ethanol has been added to the wash solution. If the binding solution turns pink due to alkalinity, add 5 μL of 3 M NaAc (pH 5.2) to adjust it back to light orange-yellow. It is recommended to use Elution Buffer (2.5 mM Tris-HCl, pH 8.5).

What should be done if elution is poor or fails?

Use a low-salt elution buffer with a pH of 8.0–8.5, accurately add the elution buffer to the center of the adsorption membrane and ensure it is fully soaked; preheating the elution buffer to 65℃ before elution can further improve the results.

What should I do if there are still small fragments or primer residues after purification?

Fragments larger than 50 bp may not completely remove primer dimers during direct purification; if there are many nonspecific bands or the template is complex, please use the Part 1 agarose gel recovery procedure.

What if the downstream enzyme digestion or ligation of recovered DNA is poor?

A common reason is ethanol residue. After washing, be sure to centrifuge the empty column at 12,000 rpm for 1 minute and leave it open for 5–10 minutes to completely remove the alcohol. If the electrophoresis shows a smeared band, it may contain ssDNA; you can renature it by heating at 95℃ for 1 minute followed by slow cooling.

13-18min

100 bp-9 kb

≥ 80%

10 μg